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INDONESIA
Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology
Published by Pusat Litbang Daya Saing Produk dan Bioteknologi Kelautan dan Perikanan
ISSN : 20895690     EISSN : 24069272     DOI : -
Core Subject : Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology
Squalen publishes original and innovative research to provide readers with the latest research, knowledge, emerging technologies, postharvest, processing and preservation, food safety and environment, biotechnology and bio-discovery of marine and fisheries. The key focus of the research should be on marine and fishery and the manuscript should include a fundamental discussion of the research findings and their significance. Manuscripts that simply report data without providing a detailed interpretation of the results are unlikely to be accepted for publication in the journal.
Arjuna Subject : -
Articles 8 Documents
 1 
Search results for , issue "Vol 14, No 1 (2019): May 2019" : 8 Documents clear
Preface Squalen Bulletin Vol. 14 No. 1 Tahun 2019 squalen Bulletin
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 14, No 1 (2019): May 2019
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v14i1.395

Abstract

Color Stability of Phycoerythrin Crude Extract (PECE) from Rhodomonas Salina Toward Physicochemical Factors Endar Marraskuranto; Tri Joko Raharjo; Rina Sri Kasiamdari; Tri Rini Nuringtyas
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 14, No 1 (2019): May 2019
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v14i1.379

Abstract

Rhodomonas salina produces Cr-phycoerythrin545 as its designated phycoerythrin (PE) with an absorption maximum at 545 nm and a shoulder 564 nm. PE has potential to be applied as colorants, pharmaceutical agents, and fluorescent dye tags. The stability of the PE color is influenced by the physicochemical factors of the solution. This study aimed to analyze the color stability of PECE against chemical (ethanol and pH) and physical (light and temperature) factors. PECE was prepared from freeze-dried biomass of R. salina and was extracted in phosphate buffer solution (pH = 6.0) using a freeze-thaw method in -25 oC (2 hours) and 4 oC (24 hours). The resulting extract was concentrated and dried in a freeze-dryer. Analyses were conducted using UV-visible and fluorescence spectrophotometer. PECE showed color stability against light of white fluorescent lamp exposure up to 8 hours, temperature exposure up to 40 oC, ethanol solution up to concentration of 20 % (v/v), and pH range 3.9-8.42. Results from this study can be useful for extraction, purification, and future application of Cr-PE545.
Front Cover Squalen Bulletin Vol. 14 No. 1 Tahun 2019 Bulletin, Squalen
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 14, No 1 (2019): May 2019
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v14i1.389

Abstract

Back Cover Squalen Bulletin Vol. 14 No. 1 Tahun 2019 Bulletin, Squalen
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 14, No 1 (2019): May 2019
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v14i1.390

Abstract

A Beginner’s Guide to Molecular Identification of Seaweed Giuseppe C. Zuccarello; Nicholas A. Paul
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 14, No 1 (2019): May 2019
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v14i1.384

Abstract

The most rapidly expanding areas for seaweed production in the world are the tropics, including Indonesia, yet these areas are also where molecular identification of local marine flora has only been sporadically employed. Furthermore, a goal for the Government of Indonesia is to diversify the types of seaweed that are being utilized, targeting valuable products and, hand in hand, to develop aquaculture techniques for these species. Morphological methods for species identification in algae are complex or unreliable, due to simple morphologies and plasticity. Therefore, it is crucial that the correct identification is made for species and varieties of commercial interest so that growth and biochemical results can be compared and contrasted between locations, across environments and over time without taxonomic ambiguity. This guide presents entry level methodologies for sample collection, DNA preservation, DNA extraction, PCR, and analyses of DNA sequence data, as a first step in the genetic characterization of both well-known cultivated species and identification of different species with potential economic properties.  
An Estimation of Endogenous Formaldehyde Exposure due to Consumption of Indonesian Opah Fish (Lampris guttatus) in Three Major Export Destination Countries Umi Anissah; Ajeng Kurniasari Putri; Giri Rohmad Barokah
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 14, No 1 (2019): May 2019
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v14i1.369

Abstract

The demand for Indonesian opah fish as an export product is increasing in the international market. Three countries (Malaysia, Mauritius, and Taiwan) recorded as the leading export destination of Indonesian opah fish. However, as the fish kept in a frozen state during export transportation, the endogenous formaldehyde may increase over time. This research presented the health risk assessment of population in the leading export destination countries that consumed opah fish from Indonesia. The study aimed to reveal the most potential export destination country that may accept an increasing volume of opah fish supply from Indonesia. The potency was determined from current export volume, the amount of endogenous formaldehyde content, and fish consumption at each country. The data were calculated with @Risk®7.0 software. The results showed opah fish consumed by Malaysian can be categorized as safe. Increasing the number of opah fish imported by Malaysian as much as six times, 12 times, 18 times, 27 and 36 times relatively does not cause health risks related to the presence of its endogenous formaldehyde. Moreover, opah fish consumed by Taiwanese is also safe, but with increasing the number of consumptions by more than 26 times is suspected to be potentially causing a health problem. However, opah fish consumed in Mauritius was categorized as unsafe and potentially caused health risks. Based on these results, Indonesia may consider to increase the opah fish export to Malaysia and Taiwan in the future.
Evaluation On Tuna Loin Handling As Raw Materials To Improve The Quality Of Frozen Tuna Loin in Ambon Theresia Dwi Suryaningrum; Diah Ikasari
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 14, No 1 (2019): May 2019
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v14i1.381

Abstract

Tuna fishing in Ambon is mostly conducted by small scale fishers using small boats (1.5 GT) and traditional fishing gear. The caught tuna was loined on board and collected at landing post or mini-plant before being sent to an UPI in Ambon. The study was aimed to evaluate the handling of tuna loin at small scale fishers during processing frozen tuna loin product. Two forms of tuna loin i.e skin-on and the skin-less tuna loin, obtained from the fishers in Latuhalat and Tial districts in Ambon and than was stored in ice overnight.  Tuna loin was  sent to Fish Processing Unit  and cleaned from the skin (for skin-on tuna loins) or trimmed (for the skin-less tuna loins). Then, tuna loins were treated with CO, wrapped with styrofoam paper, packed with plastic and incubated in chilling room at 1.12oC for 2 days until the tuna meat color becomes reddish. The tuna loins were then frozen using Air Blast Freezer (temperature of -35oC) for 8 hours. Observations were performed on the physical (temperature) and chemical properties (proximate analysis, pH and TVB), microbial contaminants (TPC, E coli and Salmonella) as well as the sensory properties of tuna loin at each processing steps, starting from   when the tuna loin was landed and handled at the collecting level,   after stored in ice overnight  before CO treatment,  after treated with CO and incubated in chilling room for 2 days before frozen tuna loin products.  The study showed that the existing tuna handling during processing  and  frozen of frozen tuna loins tended to cause quality decrease of the tuna loins. Tuna loins in the form of skin-less produced better quality compared to skin-on tuna loins. The quality decrease of tuna loin occured rapidly during incubation process after treated with CO, showed by the decrease of TVB and organoleptic values as well as the increase of bacterial content. However, the frozen tuna loin products were still meeting the requirements for frozen tuna based on Indonesia National Standard 01.2346-2006.
Screening of Marine-Derived Fungi Extracts as Antioxidant, Tyrosinase Inhibitor, and Antiglycation Muhamad Nursid; Endar Marraskuranto; Dilaika Septorini; Irmanida Batubara
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 14, No 1 (2019): May 2019
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v14i1.366

Abstract

Marine-derived fungi are of great interest as new promising sources of bioactive secondary metabolites. The aim of this study was to determine the antioxidant activity, tyrosinase inhibitor and antiglycation of marine-derrived fungi collected from Kepulauan Seribu Marine National Park, Indonesia.  Antioxidant screening was determined using the 1,1-diphenyl-2-picryl hydrazyl (DPPH) method. The tyrosinase inhibitor was screened using L-tyrosine substrate, while the antiglycation test was determined by the ability to inhibit the formation of advanced glycation end products (AGEs).  A total of 28 marine fungi isolates had been screened for their activities.  Mycelium extract of MFP 271 had the best antioxidant activity with the IC50 of 287.25 ± 50 µg/mL. Mycelium extract of MFP 277 had the best tyrosinase inhibitory activity with the IC50 of 586.42 µg/mL. The MFP 274-broth extract had the highest antiglycation activity with the IC50 value of 298.57 µg/mL. Based on the screening, the crude extracts were considered weak as antioxidant, but tyrosinase inhibitor and antiglycation activity of MFP 277 and MFP 274 are needed to investigate in depth activities.

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